Aurora (Cytek Biosciences): a full-spectrum analyser with 4 lasers, 3 scattering channels, and 48 fluorescence channels, enabling high-speed, highly sensitive single cell analysis. ImageStream X Mk II (Amnis/ Luminex): a 4-laser multispectral, imaging flow cytometer designed for the acquisition of up to 12 channels of cellular imagery. Cells may be sorted into a number of different tubes and plates. Capable of physically separating cells at rates up to 20,000 per second into ≤ 4 populations at once. FACSAria Fusion (BD): a 4-laser, 18 parameter high-speed cell sorter. This best-in-class facility is the best equipped flow core in Ireland with one state-of-the-art cell sorter (FACSAria Fusion), an imaging cytometer (ImageStream X Mark II), a full-spectrum analyser (Aurora), four flow cytometers (LSRFortessa, FACSCanto II, Accuri C6 & Attune NxT), and an in vivo fluorescence & luminescence imager (PhotonImager housed in the Comparative Medicine Unit). The Flow Cytometry Facility within the School of Biochemistry and Immunology was set up in 2005 and is located in the Trinity Biomedical Sciences Institute (TBSI). This analysis may be reasonably straightforward or elaborate for example, high-dimensional cytometry can provide up to a billion data points per single cell. 
It involves labelling cells with fluorescent probes, and requires high-value, sensitive, precision-based cellular analysers, along with post-acquisition analysis.
The technology underpins biomedical science, yielding phenotypical and functional detail in cells at rates beyond 10,000 per second. You are here Research > Cell Analysis Facilities > Flow Cytometry Facility Flow Cytometry Facility Introductionįlow cytometry is a complex technology, analysing multiple parameters on single cells at incredibly high speeds.
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